Leptin Regulates the Progression of Cell Cycle for Breast Cancer Cell Line MDA-MB-231 / 沈阳医学院学报

ABSTRACT

Objective:To study cell cycle progression induced by leptin in breast cancer cell line MDA-MB-231. Methods:MTT, cell viability test, apoptosis assays and cell cycle analysis were used to detect the effects of leptin on cell growth and proliferation of breast cancer cell line MDA-MB-231. QPCR and Western blot assay were used to assess the molecular mechanisms of leptin on cell cycle regulation of MDA-MB-231 cells. Results:When treated with 1.25 nmol/L leptin for 24 hours,the MDA-MB-231 cells were stimulated to grow obviously. With the leptin treatment,the cell viability was increased markedly and the cell death rate and apoptosis rate was decreased significantly. Cell cycle assay displayed that S phase cells increased after leptin induction. By screening the mRNA and protein levels of cell cycle regulatory factors,the results were demonstrated that leptin could speed up the transition from G1 to S phase in MDA-MB-231 cells. Conclusion:Leptin can significantly stimulate breast cancer MDA-MB-231 cell growth,strengthen the malignant ability of breast cancer cells.