Phenotypic identification of Penicillium spp. isolated from clinical wastes based on microstructure characteristics

ABSTRACT

@#Aims: The present study aimed to recognize the microstructure of conidiophores and spores of Penicilliumspp. which were isolated from clinical wastes. Methodology and results:The isolates of Penicilliumspp. were obtained from the solid clinical wastes on V8A medium and purified by single spore method. The culture characteristics were described in five culture media included; Czapek Yeast Extract Agar(CYA); Malt Extract Agar (MEA), Potato Dextrose Agar (PDA), Sabouraud Dextrose Agar (SDA) and Czapek-Dox Agar(CZ) while the conidiophores and spores were described using light and Scanning Electronic Microscope (SEM).Penicilliumspp. observedsome differences in their culture characteristics. Among 11 Penicilliumspecies isolated in this study and identified based on culture and microscope morphology. Five species including P. simplicissium,P. waksmanii,P. corylophilum andP. decumbensas well as one species identified as T. wortmanniiwere described in detailusing SEM. Conclusion, significance and impact of studyThe study revealed that the microstructure of the fungal spores and conidiophores play an important role in the taxonomy of fungi species based on the phenotypic method.