Your browser doesn't support javascript.
loading
Variant Philadelphia Chromosome Identified by Interphase Fluorescence In Situ Hybridization (FISH) without Evidence on G-banded Karyotyping and Metaphase FISH / 대한진단검사의학회지
The Korean Journal of Laboratory Medicine ; : 711-717, 2010.
Artigo em Coreano | WPRIM | ID: wpr-73296
ABSTRACT
A variant Philadelphia chromosome (Ph) is generated from translocation of one or more partner chromosomes in addition to chromosomes 9 and 22. We have described the cases of 2 patients bearing variant Ph detected by interphase FISH but not detected by G-banded karyotyping and metaphase FISH. FISH was performed using BCR/ABL dual color dual fusion translocation probes (Abbott Molecular, USA). A 52-year-old man was diagnosed with acute leukemia of mixed phenotype. G-banded karyotyping showed 46,XY,t(9;22)(q34;q11.2)[12]/47,idem,+der(22)t(9;22)[5]/46,XY[3]. Interphase FISH revealed nuc ish(ABL1,BCR)x3(ABL1 con BCRx2)[329/450]/(ABL1,BCR)x4(ABL1 con BCRx3)[5/450]/(AL1,BCR)x3(ABL1 con BCRx1)[44/450]. Metaphase FISH showed ish (9;22)(ABL1+,BCR1+;BCR+,ABL+)[22]/der(22)(BCR+,ABL1+)[3]. The other case was that of a 31-yr-old male patient diagnosed with CML in the blastic phase. G-banded karyotyping of all 20 metaphase cells showed 47,XYYc,dup(1)(q21q32),del(7)(p11.2),t(9;22)(q34;q11.2). Interphase FISH revealed nuc ish(ABL1,BCR)x3(ABL1 con BCRx2)[254/600]/(ABL1,BCR)x3(ABL1 con BCRx1)[191/600]. Metaphase FISH showed ish t(9;22)(ABL1+,BCR+;BCR+,ABL1+)[16]. These results suggest that typical t(9;22) and variant Ph may coexist in the same patient, and interphase FISH may facilitate the detection of the variant Ph that cannot be detected by G-banded karyotyping alone.
Assuntos

Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Assunto principal: Fenótipo / Translocação Genética / Cromossomos Humanos Par 9 / Cromossomos Humanos Par 22 / Cromossomo Filadélfia / Leucemia Mielogênica Crônica BCR-ABL Positiva / Leucemia / Hibridização in Situ Fluorescente / Interfase / Cariotipagem Tipo de estudo: Estudo prognóstico Limite: Adulto / Humanos / Masculino Idioma: Coreano Revista: The Korean Journal of Laboratory Medicine Ano de publicação: 2010 Tipo de documento: Artigo

Similares

MEDLINE

...
LILACS

LIS

Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Assunto principal: Fenótipo / Translocação Genética / Cromossomos Humanos Par 9 / Cromossomos Humanos Par 22 / Cromossomo Filadélfia / Leucemia Mielogênica Crônica BCR-ABL Positiva / Leucemia / Hibridização in Situ Fluorescente / Interfase / Cariotipagem Tipo de estudo: Estudo prognóstico Limite: Adulto / Humanos / Masculino Idioma: Coreano Revista: The Korean Journal of Laboratory Medicine Ano de publicação: 2010 Tipo de documento: Artigo