Study on optogenetics technology and the design of its script for virtual simulation experiment / 中华医学教育探索杂志

ABSTRACT

Optogenetics is one of the biggest breakthroughs in neurobiology in recent decades,which is a revolutionary approach to precise intervention of specific cell functions through the combination of optical and genetic engineering techniques.However,this technique involves the interdisciplinary theoretical knowledge and methods,and it requires expensive equipments and long experimental period.Thus,it is not convenient to carry out widely in experiment teaching for undergraduates except the virtual simulation teaching.In present virtual simulation experiment,we aim to learn the basic principle,operation and application of optogenetics and achieve the desired teaching effects by simulating the process of the virus package,simulating the process of the animal selection,surgery and virus stereotactic microinjection,simulating the expression process of photosensitive channel,the extracellular verification process of photosensitive channel function in vivo,and simulating the observation process that optogenetic inhibition of glutamate neurons in the amygdala affect animal fear behavior.In this paper,the content design of the script making of the virtual experiment has been discussed in the above ways.