The study of the effect of Focal adhesion kinase and p53 in rheumatoid arthritis fibroblast like synoviocytes on cell proliferation / 中华风湿病学杂志

ABSTRACT

Objective To investigate the expression of Focal adhesion kinase (FAK) and p53 in rheumatoid arthritis (RA) Fibroblast Like Synoviocytes and to explore the pathogenesis of RA.This study also studied the effect of FAK and p53 on the proliferation of Fibroblast Like Synoviocytes in RA on order to identify new target for the treatment of RA.Methods Synovial tissue from RA patients were cultured in vitro;FLS were cultured in TNF-α (10 ng/ml) and different density of protease inhibitor (MG-132) (1,5,10,20 μmol/L)for 48 h.Then the level of mRNA of both FAK and p53 in each group was tested by real time polymerase chain reaction (RT-PCR).FLS were cultured with different density of protease inhibitor (MG-132) (1,5,10,20μmol/L) for 24 h,48 h,72 h,96 h then the cell proliferation of each group were tested.ANOVA was used to compare the means between groups.Results ① After TNF-α stimulating,the level of FAK mRNA was higher than the control group [(1.48±0.09 vs(1.03±0.33),F=7.807,P＜0.05).Compared with the control group,the level of p53 mRNA decreased [(0.97±0.03) vs (1.30±0.39),F=19.933,P＞0.05).② After stimulated with different density of MG-132,the level of p53 mRNA was higher than the control group [(3.12±0.72) vs (1.30±0.39),F=19.933,P＜0.05),and the 5μ mol/L group was the highest of them.Compared with the control group,the level of FAK mRNA decreased [(0.93±0.20) vs (1.03±0.33),F=7.807,P＞0.05).③ After stimulated with different density of MG-132,the proliferation of FLS was slower than the control group (24 h F=16.654,P＜0.05;48 hF=13.652,P＜0.05;72 h F=72.999,P＜0.05;96 h F=51.533,P＜0.05).Conclusion In vitro,after stimulated with TNF-α,the level of mRNA of Focal adhesion kinase is increased,while the level of that decreases after MG-132 stimulation.Focal adhesion kinase is involved in the pathogenesis of rheumatoid arthritis.In vitro,after MG-132 stimulating,the level of mRNA of p53 is increased.p53 inhibits the excessive proliferation of RA synovial fibroblast cells and plays an important role in the pathogenesis of rheumatoid arthritis.