Role of necroptosis in liver injury in rats undergoing autologous orthotopic liver transplantation / 中华麻醉学杂志

ABSTRACT

Objective To evaluate the role of necroptosis in liver injury in rats undergoing autolo-gous orthotopic liver transplantation ( AOLT). Methods Twenty-four SPF adult male Sprague-Dawley rats, weighing 250-280 g, were divided into 3 groups ( n=8 each) using a random number table method:sham operation group ( group S ) , AOLT group ( group T ) and necroptosis inhibitor necrostatin-1 group ( group N) . Rats were anesthetized using intraperitoneal 5％ chloral hydrate 0. 5 ml∕100 g. A standard mod-el of OALT was established as previously described by Yao et al. in T and N groups. Necrostatin-11. 0 mg∕kg and the equal volume of dimethyl sulfoxide were intraperitoneally injected at 30 min before surgery in N and T groups, respectively. Blood samples were collected from the inferior vena cava at 6 h after opening the portal vein ( at 6 h after operation in group S) for determination of serum aspartate amino-transferase(AST) and alanine amino-transferase (ALT) concentrations. The livers were removed for examination of the pathological changes (with a light microscope) and for determination of the malondialdehyde (MDA) contents and superoxide dismutase ( SOD) activity ( using a spectrophotometer) and expression of receptor-interacting protein kinase-1 ( RIPK1) , RIPK3 and mixed lineage kinase domain-like ( MLKL) in liver tis-sues ( by Western blot) . The damage to livers was evaluated using Suzuki′s scoring system. Results Com-pared with group S, the serum AST and ALT concentrations, Suzuki′s score of liver tissues and MDA con-tent were significantly increased, the SOD activity was decreased, and the expression of RIPK1, RIPK3 and MLKL was up-regulated in group T ( P<0. 05) . Compared with group T, the serum AST and ALT con-centrations, Suzuki′s score of liver tissues and MDA content were significantly decreased, the SOD activity was increased, and the expression of RIPK1, RIPK3 and MLKL was down-regulated in group N ( P<0. 05) . Conclusion Necroptosis is involved in liver injury in rats undergoing AOLT.