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Caspases Activation in Ultraviolet B-induced Apoptosis of G361 Human Melanoma Cell Line
Annals of Dermatology ; : 243-246, 2000.
Artigo em Inglês | WPRIM | ID: wpr-73470
ABSTRACT

BACKGROUND:

Ultraviolet B (UVB) irradiation can induce apoptosis of melanocytes and melanoma cells. However, mechanism of UVB-induced apoptosis of melanoma cells is not clarified yet.

OBJECTIVE:

Our purpose was to study the molecular mechanism of UVB-induced apoptosis of melanoma cells.

METHODS:

G361 lightly pigmented melanoma cells were analyzed for apoptotic mechanism by flow cytometry and western blotting.

RESULTS:

G361 melanoma cells showed apoptotic features with gradual increment of UVB doses by MTT and flow cytometry. Western blotting disclosed activation of caspase-3 and poly (ADP-ribose) polymerase (PARP) after UVB irradiation.

CONCLUSION:

In this study, we showed that UVB-induced apoptosis of melanoma cells is mediated by PARP activation which is induced by caspase cascade.
Assuntos

Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Assunto principal: Linhagem Celular / Western Blotting / Apoptose / Caspases / Caspase 3 / Citometria de Fluxo / Melanócitos / Melanoma Limite: Humanos Idioma: Inglês Revista: Annals of Dermatology Ano de publicação: 2000 Tipo de documento: Artigo

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Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Assunto principal: Linhagem Celular / Western Blotting / Apoptose / Caspases / Caspase 3 / Citometria de Fluxo / Melanócitos / Melanoma Limite: Humanos Idioma: Inglês Revista: Annals of Dermatology Ano de publicação: 2000 Tipo de documento: Artigo