Effects of sin-1 on growth and cytoskeleton of endothelial cells in vitro / 第三军医大学学报
Journal of Third Military Medical University
; (24): 422-424, 2001.
Article
em Zh
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| ID: wpr-736991
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WPRO
ABSTRACT
Objective To investigate the mechanism affecting on permeability of vascular endothelial cell by nitric oxide (NO). Methods Series concentration of sin-1(a donor of NO) were added to ECV 304, a cell line of human umbilical vein endothelium. Cell growth and expression of f-actin, a cytoskeleton protein were observed. Results Cell growth was inhibited with a dose from 6.25 to 100 μmol/L and was caused to death at the concentration of 50 to 100 μmol/L by sin-1. The expression of f-actin was suppressed obviously after cultured with 100 μmol/L sin-1 for 4 hours. Conclusion It suggests that anomaly increased NO can increase permeability of blood vessels by suppressing the expression of f-actin, inhibiting cell growth or even resulting in cell death.
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Idioma:
Zh
Revista:
Journal of Third Military Medical University
Ano de publicação:
2001
Tipo de documento:
Article