Increasing the Production of IgG in Batch Tissue-culture with IgG-free Ascites / 中国生物工程杂志
China Biotechnology
; (12): 87-92, 2005.
Article
em Zh
| WPRIM
| ID: wpr-737087
Biblioteca responsável:
WPRO
ABSTRACT
An effective method has been developed for laboratory scale production of IgG. Hybridomas were cultured in serum-free media with 2% IgG-free ascites. Cell density of up to 3.55 × 10 6cells/ml and antibody concentration of 135μ g/ml after purification were abtained, which is four time more than total production of that of IgG concentration in serum-free media. This in vitro method allows great improvement in antibodies production in batch tissue culture. The method reported here is easy to handle and is economical and universally adaptable.
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Índice:
WPRIM
Idioma:
Zh
Revista:
China Biotechnology
Ano de publicação:
2005
Tipo de documento:
Article