Isolation of a Specific Antigen from the Triton X-100 Soluble Protein (TSP) Of Mycobacterium tuberculosis
Journal of Bacteriology and Virology
;
: 273-282, 2004.
Artigo
em Coreano
| WPRIM
| ID: wpr-73736
ABSTRACT
Tremendous efforts have been made to develop better vaccines and diagnostic markers for the effective control of tuberculosis. Recently, we reported that the Triton X-100 soluble protein (TSP) of Mycobacterium tuberculosis induced strong T-cell proliferation and IFN-gamma production in humans, and also conferred a significant level of protection against tuberculosis in a mouse model. In this study, the TSP was prepared by Triton X-100 extraction of Mycobacterium tuberculosis bacilli, which was followed by Triton X-114 phase partitioning. Western blot analysis using sera of 177 active pulmonary tuberculosis patients and 323 healthy individuals revealed that the TSP contained a immunodominant 40-kDa antigen specifically reacting with some sera from pulmonary tuberculosis patients. The 40-kDa antigen was purified by ion-exchange chromatography, and partially characterized by two-dimensional gel electrophoresis and N-terminal sequencing. Results of this study suggest that 40-kDa molecule of the TSP antigen from the cell suface of Mycobacterium tuberculosis can be used as a serodiagnostic marker as well as a potential vaccine candidate against tuberculosis.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Tuberculose
/
Tuberculose Pulmonar
/
Vacinas
/
Linfócitos T
/
Eletroforese em Gel Bidimensional
/
Western Blotting
/
Cromatografia por Troca Iônica
/
Octoxinol
/
Netuno
/
Mycobacterium
Limite:
Animais
/
Humanos
Idioma:
Coreano
Revista:
Journal of Bacteriology and Virology
Ano de publicação:
2004
Tipo de documento:
Artigo
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