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Comparison of results of two immunoassays for detection of hepatitis B surface antigen in pregnant women / 中华流行病学杂志
Chinese Journal of Epidemiology ; (12): 1537-1540, 2017.
Artigo em Chinês | WPRIM | ID: wpr-737869
ABSTRACT
Objective To evaluate and compare the detection consistency of hepatitis B surface antigen (HBsAg) by two immunoassays enzyme-linked immunosorbent assay (ELISA) and electrochemiluminescent immunoassay (ECLIA). Methods A prospective study was conducted among 2296 pregnant women recruited consecutively from January 1, 2014 to January 31, 2015 in a hospital. Blood samples were collected from them for the detection of HBsAg by using ELISA and ECLIA, Ka ppa test was performed on the results. Nested polymerase chain reaction and sequencing of HBV S gene were also performed in all samples. Phylogenetic analysis was performed using Mega 6.0 software. Results The two methods had high detection consistence of HBsAg ( Ka ppa=0.71). There were significant differences in detection result of B genotype and adw2 serotype HBV strains between two methods. Among 123 identified HBV strains, 113 belonged to genotype B and available for further analysis. The difference in detection of substitution rates between two methods or different positive groups were not significant. Compared with ELISA single positive group, the ECLIA single positive group had completely different substitution sites. Conclusion The two methods had high detection consistence of HBsAg, but there were still 32.4% HBV DNA positive cases in ELISA/ECLIA single positive group, and complete complementary substitution sites between ELISA single positive group and ECLIA single positive group. Our results suggested that more effective detection procedure should be considered for the possible impact of the HBV silent transmission and infection.

Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Tipo de estudo: Estudo diagnóstico / Estudo observacional Idioma: Chinês Revista: Chinese Journal of Epidemiology Ano de publicação: 2017 Tipo de documento: Artigo

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Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Tipo de estudo: Estudo diagnóstico / Estudo observacional Idioma: Chinês Revista: Chinese Journal of Epidemiology Ano de publicação: 2017 Tipo de documento: Artigo