Effects of High Glucose and Dexamethasone on the Permeability in Trabecular Meshwork Cells
Journal of the Korean Ophthalmological Society
;
: 252-260, 2018.
Artigo
em Coreano
| WPRIM
| ID: wpr-738520
ABSTRACT
PURPOSE:
To investigate the effects of high glucose (HG) and dexamethasone (DEX) on the survival and permeability of trabecular meshwork cells (HTMC), and associated changes in tight junctions.METHODS:
Primary cultured HTMC were exposed to 5 mM low glucose (LG) or 25 mM HG with or without 1.0 µM DEX for 3 days. The permeability of the HTMC monolayer was assessed using carboxyfluorescein or transendothelial electrical resistance (TEER). Gene and protein expressions of claudin-5 and occludin were assessed with reverse transcription polymerase chain reaction (RT-PCR) and Western blot, respectively.RESULTS:
HG was significantly associated with greater HTMC monolayer permeability compared to LG by both the carboxyfluorescein permeability test and TEER (p = 0.022, 0.028). HG also decreased claudin-5 and occludin mRNA expression, respectively (7.5%, 12.9%). DEX abolished HG-induced increased permeability, and increased the protein expression of claudin-5 and occludin, respectively (p = 0.015, 0.012).CONCLUSIONS:
In HTMCs, DEX reversed HG-induced permeability increase. DEX increased tight junction molecules claudin-5 and occludin. Thus, DEX-induced changes in junctional proteins could be another mechanism of increased resistance through the trabecular meshwork and may result in steroid-induced glaucoma.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Permeabilidade
/
Malha Trabecular
/
RNA Mensageiro
/
Dexametasona
/
Glaucoma
/
Western Blotting
/
Reação em Cadeia da Polimerase
/
Impedância Elétrica
/
Junções Íntimas
/
Transcrição Reversa
Idioma:
Coreano
Revista:
Journal of the Korean Ophthalmological Society
Ano de publicação:
2018
Tipo de documento:
Artigo
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