Performance of the cobas u 701 Analyzer in Urinary Tract Infection Screening
Annals of Laboratory Medicine
;
: 464-469, 2019.
Artigo
em Inglês
| WPRIM
| ID: wpr-739144
ABSTRACT
BACKGROUND:
Negative urine cultures to rule out urinary tract infections (UTI) generate a considerable laboratory workload; thus, a rapid screening test is desirable. We evaluated the performance of a new automated microscopy analyzer, cobas u 701 (Roche Diagnostics International, Rotkreuz, Switzerland) for the screening of UTI, and developed a rule-out strategy to reduce the number of samples requiring culture. We also assessed squamous epithelial cell (SEC) count as a predictor of culture contamination.METHODS:
In total, 1,604 urine samples from outpatients were analyzed with cobas u 701 and culture. Bacterial (BAC) and white blood cell (WBC) counts were used for sample interpretation. To determine a useful cut-off point to predict negative cultures, we selected the highest sensitivity and specificity values obtained from ROC curves. Diagnostic accuracy by age and gender was evaluated.RESULTS:
Urine culture showed growth of ≥104 colony forming units (CFU)/mL in 256 samples (16.0%). The highest sensitivity (91.8%) and specificity (68.4%) were obtained for cut-off points of 119 BAC/µL and 22 WBC/µL. The combination of BAC and WBC improved the performance of the rule-out strategy with a low rate of false-negative results (1.5%) and a high negative predictive value (NPV, 97.3%). Fifty-seven percent of the samples would not have required culture. SEC count was a poor predictor of culture contamination.CONCLUSIONS:
cobas u 701 can substantially reduce the number of urine samples requiring culture, with a low false-negative rate and a high NPV.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Pacientes Ambulatoriais
/
Células-Tronco
/
Sistema Urinário
/
Infecções Urinárias
/
Programas de Rastreamento
/
Curva ROC
/
Sensibilidade e Especificidade
/
Células Epiteliais
/
Leucócitos
/
Microscopia
Tipo de estudo:
Estudo diagnóstico
/
Estudo prognóstico
/
Estudo de rastreamento
Limite:
Humanos
Idioma:
Inglês
Revista:
Annals of Laboratory Medicine
Ano de publicação:
2019
Tipo de documento:
Artigo
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