Biphasic Increase of Pro-inflammatory Cytokines in Mice Lung after Irradiation / 결핵및호흡기질환
Tuberculosis and Respiratory Diseases
;
: 14-20, 2009.
Artigo
em Coreano
| WPRIM
| ID: wpr-74000
ABSTRACT
BACKGROUND:
The pathophysiologic mechanisms of radiation-induced lung injury should be elucidated to enhance the therapeutic efficacy of radiotherapy and to manage patients exposed to serious radiation by accident. It has been suggested that pro-inflammatory cytokines play an important role in radiation-induced effect on the lung. This study was aimed to investigate changes in pro-inflammatory cytokines such as TNF-alpha, MIP-2, IL-1beta and HMGB1, a newly recognized inflammatory mediator.METHODS:
The chests of BALB/c mice were selectively irradiated with single fraction of 20 Gy and then sacrificed at indicated times. Pathologic changes in the lung were examined after H&E staining. The expression level of pro-inflammatory cytokines was evaluated by ELISA kits in lung homogenate and in serum.RESULTS:
Radiation induced inflammatory changes and mild fibrosis in lung. Biphasic increase of TNF-alpha and IL-1beta was found in lung homogenate at 4 hours and at 3 weeks after radiation. The elevation in the second phase tended to be more intense. However, there was no similar change in serum. MIP-2 level was slightly increased in lung homogenate at 4 hours, but not at 3 weeks. HMGB1 was increased at 3 weeks in serum while there was no significant change in lung homogenate.CONCLUSION:
Radiation induced a biphasic increase in TNF-alpha and IL-1beta. The effective control of second phase cytokine elevation should contribute to preventing severe lung fibrosis caused by radiation.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Tórax
/
Fibrose
/
Ensaio de Imunoadsorção Enzimática
/
Citocinas
/
Fator de Necrose Tumoral alfa
/
Proteína HMGB1
/
Lesão Pulmonar
/
Pulmão
Limite:
Animais
/
Humanos
Idioma:
Coreano
Revista:
Tuberculosis and Respiratory Diseases
Ano de publicação:
2009
Tipo de documento:
Artigo
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