Effect of IL-6 on fibronectin in bovine trabecular meshwork cells cultured <i>in vitro</i> / 国际眼科杂志(Guoji Yanke Zazhi)
International Eye Science
;
(12): 911-915, 2019.
Artigo
em Chinês
| WPRIM
| ID: wpr-740486
ABSTRACT
@#AIM:
To investigate the changes of fibronectin expression in cultured bovine trabecular meshwork cells when cells were stimulated by different concentrations of IL-6 to provide evidence for early diagnosis and new therapy of POAG.<p>METHODS:
We identify third-generation bovine trabecular meshwork cells which were got from tissue mass culture method. Then the relative expression of FN gene and protein in cells were detected by Real-Time PCR and Western-blot after 24h stimulation with 0ng/mL, 0.1ng/mL, 0.5ng/mL IL-6.<p>RESULTS:
The cultured bovine trabecular cells are coincident with what recorded in the book. Real-time PCR and Western blot showed that the amount of FN mRNA produced by cells was 1.000±0.000, 0.213±0.004, 0.056±0.001, 0.019±0.002 respectively, and the protein expression was 1.167±0.012, 0.662±0.009, 0.238±0.011, 0.061±0.011 respectively. There was a significant difference among four groups(<i>P</i><0.05).<p>CONCLUSION:
Cultured bovine trabecular meshwork cells have a negative correlation with the expression of FN protein after being stimulated by exogenous IL-6, and the results are consistent with the actual state of the disease. We speculate that the IL-6 participate in the pathogenesis and progression of POAG by affecting the expression of FN gene and protein and changing the structure of trabecular meshwork.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Tipo de estudo:
Estudo de rastreamento
Idioma:
Chinês
Revista:
International Eye Science
Ano de publicação:
2019
Tipo de documento:
Artigo
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