Identification and HPLC Quantification of a Phytoecdysone and Three Phenolic Glycosides in Lamium takesimense Nakai
Natural Product Sciences
;
: 241-246, 2018.
Artigo
em Inglês
| WPRIM
| ID: wpr-741635
ABSTRACT
The herbs of Lamium takesimense Nakai (Lamiaceae) is used to treat spasmodic and inflammatory disease. The four polar compounds, ecdysterone, isoacteoside, rutin and lamiuside C, were isolated and identified from the BuOH fraction of the L. takesimense MeOH extract. HPLC quantification was performed on a Capcell Pak C18 column (5 µm, 4.6 mm × 250 mm) with a gradient elution of H₂O and 0.05% acetic acid in MeOH. The HPLC method was validated in terms of linearity, sensitivity, stability, precision, and accuracy. The quantitative level in plant material was determined as the following order lamiuside C (4, 3.75 mg/g dry weight) > ecdysterone (1, 1.93 mg/g) > isoacteoside (2, 1.32 mg/g) > rutin (3, 0.97 mg/g).
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Plantas
/
Rutina
/
Cromatografia Líquida de Alta Pressão
/
Ácido Acético
/
Lamiaceae
/
Fenol
/
Ecdisona
/
Ecdisterona
/
Glicosídeos
/
Métodos
Tipo de estudo:
Estudo diagnóstico
Idioma:
Inglês
Revista:
Natural Product Sciences
Ano de publicação:
2018
Tipo de documento:
Artigo
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