Orexin-A inhibits capsaicin-induced changes in cyclooxygenase-2 and brain-derived neurotrophic factor expression in trigeminal nucleus caudalis of rats
The Korean Journal of Pain
;
: 174-182, 2018.
Artigo
em Inglês
| WPRIM
| ID: wpr-742190
ABSTRACT
BACKGROUND:
The trigeminal nucleus caudalis (Vc) is a primary central site for trigeminal transmitting. Noxious stimulation of the trigeminal nociceptors alters the central synaptic releases and neural expression of some inflammatory and trophic agents. Orexin-A and the orexin 1 receptor (OX1R) are expressed in pain pathways including trigeminal pain transmission. However, the the mechanism(s) underling orexin-A effects on trigeminal pain modulation have not been fully clarified.METHODS:
Trigeminal pain was induced by subcutaneous injection of capsaicin in the upper lip in rats. The effect of trigeminal pain on cyclooxygenase-2 (COX-2) and brain-derived neurotrophic factor (BDNF) expression in the Vc of animals was determined by immunofluorescence. Subsequently, OX1R agonist (orexin-A) and antagonist (SB-334867-A) was administrated in the Vc to investigate the possible roles of the Vc OX1R on changes in COX-2 and BDNF levels following pain induction.RESULTS:
The data indicated an increase in COX-2 and decrease in BDNF immuno-reactivity in the Vc of capsaicin, and capsaicin- pretreated with SB-334867-A (80 nM), groups of rat. However, the effect of capsaicin on COX-2 and BDNF expressions was reversed by a Vc microinjection of orexin-A (100 pM).CONCLUSIONS:
Overall, the present data reveals that orexin-A can attenuate capsaicin-induced trigeminal pain through the modulation of pain effects on COX-2 and BDNF expressions in the Vc of rats.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Núcleo Inferior Caudal do Nervo Trigêmeo
/
Neuralgia do Trigêmeo
/
Núcleos do Trigêmeo
/
Medição da Dor
/
Dor Facial
/
Nociceptores
/
Capsaicina
/
Imunofluorescência
/
Fator Neurotrófico Derivado do Encéfalo
/
Ciclo-Oxigenase 2
Limite:
Animais
Idioma:
Inglês
Revista:
The Korean Journal of Pain
Ano de publicação:
2018
Tipo de documento:
Artigo
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