Detection of Cryptosporidium spp. in Diarrheic Immunocompetent Patients in Beni-Suef, Egypt: Insight into Epidemiology and Diagnosis
The Korean Journal of Parasitology
;
: 113-119, 2018.
Artigo
em Inglês
| WPRIM
| ID: wpr-742242
ABSTRACT
Cryptosporidium species is an important cause of gastrointestinal infections globally. This study aimed to shed light on its role in diarrheic immunocompetent patients in Beni-Suef, Egypt and to compare three diagnostic methods. Two hundred diarrheic patients, 37±16.8 year old, were enrolled. Stool samples were examined by light microscopy, using modified Ziehl-Neelsen stain (MZN) for Cryptosporidium spp. oocysts. Coproantigens were detected by sandwich ELISA. DNA molecular diagnosis was done by nested PCR. PCR yielded the highest detection rates (21.0%), compared to ELISA (12.5%) and MZN staining method (9.5%). The higher infection rates were in 20–40 year-old group, followed by 40–60 year-old. Association between epidemiologic factors was statistically not significant; positivity and gender, clinical manifestations, residence, source or water, or contact with animals. Cryptosporidiosis is an important enteric parasitic infection in Beni-Suef and PCR remains the gold standard for diagnosis.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
DNA
/
Ensaio de Imunoadsorção Enzimática
/
Água
/
Fatores Epidemiológicos
/
Reação em Cadeia da Polimerase
/
Epidemiologia
/
Criptosporidiose
/
Cryptosporidium
/
Oocistos
/
Diagnóstico
Tipo de estudo:
Estudo diagnóstico
/
Estudo de rastreamento
Limite:
Animais
/
Humanos
País/Região como assunto:
África
Idioma:
Inglês
Revista:
The Korean Journal of Parasitology
Ano de publicação:
2018
Tipo de documento:
Artigo
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