Effect of silencing mitochondrial ribosomal protein L35 gene on growth of human esophageal cancer TE-1 cells / 吉林大学学报(医学版)
Journal of Jilin University(Medicine Edition)
;
(6): 28-32,后插1, 2019.
Artigo
em Chinês
| WPRIM
| ID: wpr-742722
ABSTRACT
Objective:
To investigate the effect of lentivirus-mediated silencing of mitochondrial ribosomal protein L35 (MRPL35) gene on the growth of human esophageal cancer TE-1cells, and to clarify its mechanism.Methods:
Three kinds of human esophageal cancer cells, TE-1, ECA109and KYSE150, were selected.The relative expression levels of MRPL35mRNA in three kinds of cells by real-time quantitative PCR.The esophageal cancer TE-1cells were divided into shMRPL35group and shCtrl group, and the cells were infected with si-RNA lentivirus and si-RNA lentivirus;the esophageal cancer cell line stably silenting the MRPL35gene was established.Real-time quantitative PCR and Western blotting methods were used to detect the efficiency of MRPL35gene silencing.The cell growth curves in various groups were detected by CCK-8method, and the apoptotic rates were detected by flow cytometry after AnnexinⅤ-PE/7AAD double staining.Results:
Three kinds of esophageal cancer cells expressed MRPL35gene, and the expression levels were not statistically significant between them (P>0.05) .The results of real-time quantitative PCR and Western blotting methods showed that the mRNA and protein levels of MRPL35in the TE-1cells in shMRPL35group were significantly lower than those in shCtrl group (P<0.05) .Compared with shCtrl group, the cell growth speed in shMRPL35group was decreased (P<0.05) , and the apoptotic rate was significantly increased (P<0.01) .Conclusion:
Silencing MRPL35gene can inhibit the proliferation of esophageal cancer TE-1cells and plays a role through the apoptotic pathway.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Idioma:
Chinês
Revista:
Journal of Jilin University(Medicine Edition)
Ano de publicação:
2019
Tipo de documento:
Artigo
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