Study on the level of neutrophil autophagy in peripheral blood of patients with tuberculosis infection / 国际检验医学杂志

ABSTRACT

Objective To investigate the autophagy level of peripheral blood neutrophils in patients with tuberculosis infection.Methods According to the standard for tuberculosis diagnosis, the subjects were divided into normal control group (16 cases) and active tuberculosis group (24 cases), and peripheral blood was collected.The autophagy of neutrophils and mononuclear cells of the two groups were detected by flow cytometryThe total RNA of neutrophils was extracted by Trizol and the relative quantification of Beclin1 expression was calculated by using 2-△△Ct in real-time quantitative PCR.Results There was no significant difference in age between the active tuberculosis and normal control group (P=0.165 5, P＞0.05);the proportion of male in active tuberculosis group (79.2％) was significantly higher than that of normal control group (37.5％), and the difference was statistically significant (P=0.031 5, P＜0.05).The level of neutrophil autophagy in the active tuberculosis group was significantly lower than that of the normal control group (P=0.013 8, P＜0.05), and there was no difference of mononuclear cells between the two groups (P=0.784 2, P＞0.05);active tuberculosis group the mRNA expression of Beclin1△ Ct=-1.254±0.40 was lower than that of the normal control group△Ct=-0.10±0.48, the difference was statistically significant (P＜0.001).Conclusion The incidence of active tuberculosis in male is higher than that of female;the autophagy level of neutrophils in peripheral blood of patients with tuberculosis infection decreased, and the decline of autophagy-related gene Beclin1 mRNA expression may be related to the occurrence and development of tuberculosis.