Effects of resveratrol on proliferation and invasion of lung cancer 95D cells / 国际肿瘤学杂志

ABSTRACT

Objective To study the effects of resveratrol on the proliferation and invasion capacity of human lung adenocarcinoma 95D cells.Methods Human lung adenocarcinoma 95D cells were treated with 0,10,20,40,80 μmol/L resveratrol and treated with 0 μmol/L as the control group.The proliferation level of 95D cells was measured by methyl thiazolyl tetrazolium (MTT).Cell cycle and apoptosis were detected by flow cytometry.Cell adhesion rate was determined by in vitro adhesion test.Cell invasiveness was measured by Transwell chamber.The expressions of matrix metalloproteinase-2 (MMP-2) and tissue inhibitior of metalloproteinase-2 (TIMP-2) were determined by fluorescent immunocytochemistry.Results When the 95D cells were treated with resveratrol for 72 h,the cell proliferation rates in groups treated with 0,10,20,40,80 μmol/L resveratrol were 100％,(82.23 ±0.33)％,(62.45±0.27)％,(49.89±0.43)％,(45.11 ±0.35)％ respectively,with a significant difference (F =87.830,P =0.002).The proliferation rates of 95D cells in the10,20,40,80 μmol/L resveratrol groups were significantly inhibited compared with the control group (P =0.017,P ＜0.001,P ＜0.001,P ＜0.001).When the 95D cells were treated for 48 h,the apoptosis rates of cells in each group were 0,(34.90 ±0.91)％,(41.33 ±0.13)％,(45.47 ±0.87)％,(59.46 ±0.59)％ respectively,with a significant difference (F =21.032,P =0.002).The apoptosis rates of 95D cells in the 10,20,40,80 μmol/L resveratrol groups were significantly increased compared with the control group (P =0.001,P ＜0.001,P ＜0.001,P ＜0.001).When the 95D cells were treated for 48 h,the S phase cell percentages in each group were (18.12 ±0.62)％,(38.33 ±0.62)％,(54.15 ±0.74)％,(44.85 ± 0.82)％,(50.01 ±0.35)％ respectively,with a significant difference (F =104.156,P =0.001).The S phase cell percentages in the 10,20,40,80 μmol/L resveratrol groups were significantly higher compared with the control group (P =0.001,P ＜ 0.001,P ＜ 0.001,P ＜ 0.001).When the 95D cells were treated for 24 h,the cell adhesion rates in each group were 100％,(87.41 ± 0.02) ％,(84.32 ± 0.03) ％,(68.23 ± 0.04)％,(63.01 ±0.02)％ respectively,with a significant difference (F=13.760,P＜0.001).The cell adhesion rates in the 20,40,80 μmol/L resveratrol groups were significantly inhibited compared with the control group (P =0.035,P ＜ 0.001,P ＜ 0.001),while the 10 μmol/L resveratrol group had no significant difference compared with the control group (P =0.058).When the 95D cells were treated for 24 h,the cell invasion rates in each group were 100％,(97.01 ± 0.03) ％,(74.89 ± 0.07) ％,(34.07 ± 0.03) ％,(14.65 ±0.02)％ respectively,with a significant difference (F =39.382,P =0.001).The cell invasion rates in the 20,40,80 μmol/L groups were significantly inhibited compared with the control group (P =0.012,P ＜ 0.001,P ＜ 0.001),while the 10 μmol/L resveratrol group had no significant difference compared with the control group (P =0.881).When the 95D cells were treated for 48 h,MMP-2 protein expression was decreased in the 20 mol/L group,while TIMP-2 protein expression was increased compared with the control group.Conclusion Resveratrol can inhibit the proliferation of human lung adenocarcinoma 95D cells and has effect of anti-tumor cell invasiveness,and its mechanism may involve two-way regulation of MMP-2/TIMP-2 expression.