Abnormality of resting-state functional network in a rat animal model of sepsis-associated encephalopathy / 临床麻醉学杂志

ABSTRACT

Objective To explore changes in the functional network in an animal model of SAE induced by lipopolysaccharide (LPS) challenge.Methods Thirty male Sprague-Dawley rats were randomly allocated into control group and group SAE.SAE model was established by intraperitoneal injection of LPS 1 mg/kg in group SAE, while the same amount of saline.was injected in the control group.Firstly, we used regional homogeneity (ReHo) method to exam abnormal brain regions between the control and SAE groups and then considered them as seeds for functional connectivity analysis, and behavioral tests were performed at 48 hours after LPS intraperitoneal injection.Results Compared with the control group, our study showed LPS impaired mood function, as reflected by depression-like behavior of prolonged immobility[ (38.93± 13.84) s vs (22.06± 6.75) s, P<0.05]in the forced swim test;LPS induced significantly increased ReHo values in the anterior cingulate cortex (ACC) and caudate putamen (CPu) of (1.21±0.07 vs 0.97±0.12, P<0.05) and (1.34±0.09 vs 1.17±0.16, P<0.05) respectively;the ACC seed showed increased functional connectivity with the retrosplenial cortex (0.45 ± 0.06 vs 0.11 ± 0.02, P<0.05) ;the right CPu seed showed increased functional connectivity with the left Cpu (0.33±0.07 vs 0.07±0.01, P<0.05) ;the depression-like behavior was associated with increased ReHo values in the ACC and right CPu as well as increased functional connectivity between the right CPu and left CPu.Conclusion This study suggests that the impaired mood function in SAE is associated with increased ReHo values in the ACC and right CPu as well as increased functional connectivity between the right CPu and left CPu, indicating that the abnormality of resting-state functional network may be a potential mechanism involved in SAE.