Effects of sevoflurane on proliferation, invasion, apoptosis and chemosensitivity of osteosarcoma cells / 临床麻醉学杂志

ABSTRACT

Objective To investigate effects of sevoflurane on proliferation, invasion, apoptosis and chemosensitivity of osteosarcoma cells. Methods The osteosarcoma MG63 cells were randomly divided into control group, 1.7％ sevoflurane group, 3.4％ sevoflurane group and 5.1％ sevoflurane group. The control group did not receive sevoflurane, and the rest were given with 1.7％, 3.4％, and 5.1％ concentrations sevoflurane, respectively. Cell proliferation was detected by MTT, cell apoptosis was detected by flow cytometry and cell migration was detected by Transwell cell invasion test. Each group was treated with cisplatin, and the apoptosis of each group was detected. Results The OD values of 1.7％ sevoflurane group, 3.4％ sevoflurane group and 5.1％ sevoflurane group at 24 h, 36 h and 72 h after cultured were significantly lower than that of the control group (P ＜ 0.05), of which OD values in 5.1％ sevoflurane group at 24 h, 36 h and 72 h after cultured were significantly less than 1.7％ sevoflurane group, 3.4％ sevoflurane group (P ＜ 0.05). There were no significant difference in cell apoptosis rate, cell migration number of the four group. Under the effect of cisplatin, the apoptosis rate in 1.7％ sevoflurane group, 3.4％ sevoflurane group and 5.1％ sevoflurane group were significantly lower than that of the control group (P ＜ 0.05), of which apoptosis cell rate in 5.1％ sevoflurane group were significantly lower than that of the 1.7％ sevoflurane group and 3.4％ sevoflurane group (P ＜ 0.05). Conclusion Sevoflurane, inhibiting the proliferation of osteosarcoma MG63 cells, has no significant effect on cell migration and apoptosis and reduces the sensitivity of osteosarcoma MG63 cells to cisplatin.