Expression of BMP-4 in prostate cancer tissues and its significance / 临床与实验病理学杂志

ABSTRACT

Purpose To explore the expression of bone morphogenetic protein-4 (BMP-4) in prostate cancer tissues and to analyze the effects of its down-regulation on proliferation and invasion of prostate cancer cells. Methods The expression of BMP-4 protein in prostate tissues of 58 patients with prostate cancer and 40 patients with benign prostatic hyperplasia (BPH) was detected by immunohistochemical En Vision method. The BMP-4 protein expression of different prostate cancer cells (LNcap, PC-3, Du145) and BPH was analyzed by Western blot.BMP-4 siRNA and control siRNA were transfected into LNcap respectively and then divided into three groups untreated group, control siRNA group and BMP-4 siRNA group. Western blot was used to detect the expression levels of BMP-4 protein, MMP-2and MMP-9 in LNcap cells among the three groups. The proliferation of three groups was detected by CCK 8. The invasion ability of three groups was detected by Transwell chamber. Results The positive proportion of BMP-4 protein in prostate cancer tissues was higher than that in benign prostatic hyperplasia (P < 0.05) , and the expression level of BMP-4 protein in three kinds of prostate cancer cells was higher than that in BPH cell. The expression of BMP-4 protein in BMP-4 siRNA group was lower than that in untreated group and control siRNA group.The cell proliferation ability and cell invasion ability in BMP-4siRNA group were lower than those in untreated group and control siRNA group. The expression levels of MMP-2 and MMP-9proteins in BMP-4 siRNA group were lower than those in untreated group and control siRNA group. Conclusion BMP-4 is highly expressed in prostate cancer tissues and is associated with proliferation and invasion of prostate cancer cells, and it may become a potential therapeutic target for prostate cancer.