Effects of TMEM16F on the Proliferation and Migration of Breast Cancer Cells / 中国医科大学学报

ABSTRACT

Objective To investigate the expression of transmembrane protein 16 F (TMEM16 F) in breast cancer cells and to elucidate the effect of the knockdown of its gene on proliferation and migration of T47 D breast cancer cells. Methods The expression of TMEM16 F protein in MDA-MB-231 and T47 D breast cancer cells was detected by Western blotting. The effect of TMEM16 F knockdown on the proliferation of T47 D breast cancer cells was detected by CCK-8 cell proliferation assay, and its effect on the migration of T47 D breast cancer cells was examined by cell scratch assay. Results Western blotting results showed TMEM16 F protein expression in breast cancer cells.The results of CCK-8 cell proliferation assay showed that compared to the Scrambled shRNA group, the proliferation of T47 D breast cancer cells was decreased after TMEM16 F knockdown (P = 0.037 0). The results of the scratch test showed that the migration ability of T47 D breast cancer cells was enhanced after TMEM16 F knockdown, compared to the control group (P = 0.002 7). Conclusion TMEM16 F is highly expressed in T47 D breast cancer cells. Knockdown of TMEM16 F can inhibit the proliferation and promote the migration of T47 D breast cancer cells.