Comparison of adriamycin-resistant breast cancer lines induced in two ways / 中国医师杂志

ABSTRACT

Objective To compare the biological characteristics of two Adriamycin-resistant breast cancer lines induced in different methods.Methods The MCF-7/ADM1 and MCF-7/ADM2 of breast cancer resistant cell lines were induced by low-concentration continuous increasing method and high concentration impact increasing method respectively.The morphological characteristics of the two cell lines were observed microscopically.The resistance index of the cells was determined with methyl thiazolyl tetrazolium (MTT) assay.The cell growth curves were drawn,and calculate the cell double time.Cryopreservation in different concentrations of adriamycin (ADM),the resistant index (RI) changes were detected and the cell cycle was detected by flow cytometry.Results We succeeded in inducing drug-resistant breast cancer cell lines,which were cross-resistance to paclitaxel and cisplatin.The RI of MCF-7/ADM1 was higher than that of MCF-7/ADM2 (P ＜ 0.05).The Td of three cell lines were 24.01 h,34.04 h and 32.71 h,respectively.The RI of the two drug-resistant cell lines did not change significantly after cryopreservation (P ＞ 0.05).Compared with MCF-7 cell line,cells of drug-resistant cell lines decreased in S,G2/M phase,while that in G0/G1 phase increased.Conclusions The two methods can induce drug-resistant cell lines,and the resistance was relatively stable,but its biological characteristics were partially different.Its resistance mechanism and reversal target need to be further studied.