Preliminary mechanism of paclitaxel enhanced radiation sensitivity for nasopharyngeal carcinoma cells / 临床耳鼻咽喉头颈外科杂志
Journal of Clinical Otorhinolaryngology Head and Neck Surgery
; (24): 1129-1136, 2014.
Article
em Zh
| WPRIM
| ID: wpr-746485
Biblioteca responsável:
WPRO
ABSTRACT
OBJECTIVE@#To study the radiation-sensitizing function and preliminary mechanism of paclitaxel in radiation-resistant nasopharyngeal carcinoma cells.@*METHOD@#X-ray dose fractionated irradiation technology to build radiation-resistant subline of nasopharyngeal carcinoma; CNE-2S1 was treated with paclitaxel alone or combined with radiation therapy, while control group treated with radiation therapy; cell colony formation assay was used to observe sensitizing effect of paclitaxel on radiotherapy; flow cytometry analysis was used to analyze cell cycle distribution and apoptosis ratio of different treatment groups; immunoblotting was used to analyze SHP-1 expression levels of different treatment groups.@*RESULT@#Nasopharyngeal carcinoma cells resistant to radiation was successfully established; cell colony formation assay showed that paclitaxel has obvious sensitizing effect on radiotherapy; FACS results showed that: CNE-2S1 treated by paclitaxel were arrested in G2M phase; paclitaxel and radiotherapy treatments significantly improved the CNE-2S1 apoptosis ratio; Western blot results showed that paclitaxel and combined radiotherapy can reduce the CNE-2S1 cells SHP-1 expression levels.@*CONCLUSION@#Paclitaxel enhanced radiation therapy for nasopharyngeal carcinoma cells resistant to radiation, and SHP-1 may be involved in this progress.
Texto completo:
1
Índice:
WPRIM
Assunto principal:
Patologia
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Farmacologia
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Tolerância a Radiação
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Carcinoma
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Ciclo Celular
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Neoplasias Nasofaríngeas
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Paclitaxel
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Apoptose
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Linhagem Celular Tumoral
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Proteína Tirosina Fosfatase não Receptora Tipo 6
Tipo de estudo:
Diagnostic_studies
Limite:
Humans
Idioma:
Zh
Revista:
Journal of Clinical Otorhinolaryngology Head and Neck Surgery
Ano de publicação:
2014
Tipo de documento:
Article