The study on growth and proliferation of neural stem cells from rats in vitro / 临床耳鼻咽喉头颈外科杂志
Journal of Clinical Otorhinolaryngology Head and Neck Surgery
;
(24): 747-750, 2008.
Artigo
em Chinês
| WPRIM
| ID: wpr-746592
ABSTRACT
OBJECTIVE@#To investigate culturing neural stem cells (NSCs) from rat embryos in vitro and to observe their growth and differentiation.@*METHOD@#NSCs were isolated from hippocampus of SD rat embryos (P16-P18) and cultured in DMEM/F12 medium containing EGF, bFGF, B27. To observe process of cell proliferation by microscope and identify cell types by immunocytochemical analyses after differentiation.@*RESULT@#NSCs grew well in serum-free conditional medium and their cell bodies present transparent with good refraction at about eighth day. After differentiation, the cells demonstrated NSE and GFAP immunoreactive.@*CONCLUSION@#NSCs were cultured well in serum-free conditional medium and they could be induced to differentiate into neurons and astrocytes in serum conditional medium.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Diferenciação Celular
/
Células Cultivadas
/
Embriologia
/
Meios de Cultura Livres de Soro
/
Ratos Sprague-Dawley
/
Biologia Celular
/
Células-Tronco Multipotentes
/
Proliferação de Células
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Embrião de Mamíferos
/
Células-Tronco Neurais
Limite:
Animais
/
Gravidez
Idioma:
Chinês
Revista:
Journal of Clinical Otorhinolaryngology Head and Neck Surgery
Ano de publicação:
2008
Tipo de documento:
Artigo
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