Stat3 antisense oligodeoxynucleotide induces apoptosis in laryngeal carcinoma cells by regulating apoptosis-related factors / 临床耳鼻咽喉头颈外科杂志

ABSTRACT

OBJECTIVE@#To study the mechanism of apoptosis in laryngeal carcinoma cell induced by Stat3 antisense oligodeoxynucleotide (ASODN).@*METHOD@#The designed Stat3 ASODN was transferred into laryngeal carcinoma Hep-2 cell by lipofection. Expression of Bcl-2, Bax and C-Myc were detected by Western blot and PCR.@*RESULT@#Western blot and PCR results demonstrated that Stat3 ASODN could significantly increased the expression of Bax and decreased the expression of Bcl-2 and C-Myc when the concentration of antisense oligodeoxynucleotide were heightened.@*CONCLUSION@#Stat3 ASODN participate in apoptosis by enhancing the expression of Bax and reducing the expression of Bcl-2 and C-Myc.