Analysis of proteomic spectra in serum from patients with laryngeal carcinoma by SELDI-TOF-MS technology / 临床耳鼻咽喉头颈外科杂志
Journal of Clinical Otorhinolaryngology Head and Neck Surgery
;
(24): 1116-1119, 2009.
Artigo
em Chinês
| WPRIM
| ID: wpr-746691
ABSTRACT
OBJECTIVE@#To screen the tumor markers of laryngeal carcinoma and to investigate their expression in preoperative and postoperative serum.@*METHOD@#The distinct protein in serum was detected in 32 cases of laryngeal carcinoma and 38 healthy people by IMAC-Cu proteinchip array and surface-enhanced laser desorption/ionization time-of-flight mass spectrometry (SELDI-TOF-MS). The distinct proteins in serum were detected in 32 cases of laryngeal carcinoma preoperation and within 10 days 15 cases of laryngeal carcinoma postoperation with the same methods. The discriminatory profiling between preoperative and postoperative patients was analyzed by Biomarker Wizard software and Biomarker Pattern software.@*RESULT@#The results showed that fifteen differentially expressed proteins in serum were screened by analysis of proteomic spectra of preoperative patients and normal subjects. Seventeen kinds of protein differentially expressed in serum were screened by analysis of proteomic spectra of preoperative and postoperative patients. Six kinds of protein (2958.52, 3796.89, 5148.86, 6115.57, 4052.18, and 7770.76) were obtained for making up patterns that was able to class the preoperative-team and postoperative-team. Corresponding correct ratio were 84.38% (27/32) and 73.33% (11/15).@*CONCLUSION@#The preliminary results suggest that classification system will provide a highly accurate and innovative approach for the early diagnosis of laryngeal carcinoma and judgement of prognosis. SELDI-TOF-MS technology is a useful tool for a high throughput screening of large-sized serum samples to discover potential biomarkers for laryngeal carcinoma.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Patologia
/
Prognóstico
/
Sangue
/
Biomarcadores Tumorais
/
Neoplasias Laríngeas
/
Espectrometria de Massas por Ionização e Dessorção a Laser Assistida por Matriz
/
Análise Serial de Proteínas
/
Métodos
Tipo de estudo:
Estudo prognóstico
/
Estudo de rastreamento
Limite:
Idoso
/
Feminino
/
Humanos
/
Masculino
Idioma:
Chinês
Revista:
Journal of Clinical Otorhinolaryngology Head and Neck Surgery
Ano de publicação:
2009
Tipo de documento:
Artigo
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