Liver Non-Parenchymal Cells Induce Apoptosis in Activated T Cells in Vitro / 대한이식학회지
The Journal of the Korean Society for Transplantation
;
: 73-78, 2001.
Artigo
em Inglês
| WPRIM
| ID: wpr-74674
ABSTRACT
PURPOSE:
Liver, unlike heart or skin, allografts transplanted between MHC-disparate mouse strains are spontaneously accepted without any immunosuppressive therapy. Despite the allograft acceptance, the recipients continue to exhibit donor-specific immune responses in vitro (MLR and generation of CTL). High levels of CTL apoptosis evident within tolerated liver grafts have been postulated as a mechanism underlying this 'split' tolerance. METHODS andRESULTS:
By using radiometric DNA fragmentation test ("JAM" assay) and TUNEL staining, we present the evidence here that liver nonparenchymal cells (NPC) are quite strong inducers of activated T cell apoptotic death in allogeneic mice. This phenomenon occurs the similar level in activated T cells of syngeneic or third-party mice. Liver cells from gld (FasL-deficient) mice exert similar apoptosis-inducing effect on activated T cells from normal mice. Tumor necrosis factor receptor (TNFR) Fc fusion protein, and concanamycin A, an inhibitor of perforin pathway, fail to inhibit the apoptotic activity.CONCLUSION:
These data indicate that liver NPC play important role in causing active apoptosis in graft-infiltratingCTL which favors liver graft acceptance, and liver-induced activated T cell apoptosis may not mediated by Fas, TNF or perforin pathways.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Pele
/
Transplante
/
Linfócitos T
/
Apoptose
/
Receptores do Fator de Necrose Tumoral
/
Transplantes
/
Marcação In Situ das Extremidades Cortadas
/
Fragmentação do DNA
/
Perforina
/
Aloenxertos
Limite:
Animais
Idioma:
Inglês
Revista:
The Journal of the Korean Society for Transplantation
Ano de publicação:
2001
Tipo de documento:
Artigo
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