FA-MNP-MMP-9-ASODN complex construction and the study of its FA molecular targeting ability / 临床耳鼻咽喉头颈外科杂志
Journal of Clinical Otorhinolaryngology Head and Neck Surgery
;
(24): 593-597, 2013.
Artigo
em Chinês
| WPRIM
| ID: wpr-747064
ABSTRACT
OBJECTIVE@#To construct FA targeted magnetic nanocomplex (FA-MNP-MMP-9-ASODN) loading matrix metalloproteinase 9 (MMP-9) antisense oligonucleotide (ASODN) and evaluate its targeting capacity and efficiency of gene transfection to folate receptor (FR) positive NPC.@*METHOD@#FA-MNP-MMP-9-ASODN was constructed by MMP-9-ASODN coupling with FA-MNP prepared by our research team through the aldehyde-ammonia condensation reaction. To analyze the feasibility of ASODN coupling with nanocarrier agarose gel electrophoresis. Two kinds of HNE-1 and CNE-2 cells and implanted tumors phagocytosis of FA-MNP-MMP-9-ASODN were observed by MRI on tumor-bearing nude mice, iron staining and TEM. To analyze gene transfection of the vector by observing FITC in the cell.@*RESULT@#The electrophoresis results revealed ASODN successfully coupling with FA-MNP. HNE-1 cell can effectively ingest the nanocomposite,with more FITC in the cell, but CNE-2 cell had not uptake for the nanocomposite, with no FITC in the cell. By comparing with CNE-2 tumor, HNE-1 tumor also can efficiently swallow the nanocomposite.@*CONCLUSION@#FA-MNP-MMP-9-ASODN nanocomplex is constructed successfully with good FA targeting ability and gene transfection.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Transfecção
/
Oligonucleotídeos Antissenso
/
Metaloproteinase 9 da Matriz
/
Nanocompostos
/
Ácido Fólico
/
Vetores Genéticos
/
Genética
/
Camundongos Endogâmicos BALB C
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Camundongos Nus
/
Transplante de Neoplasias
Limite:
Animais
Idioma:
Chinês
Revista:
Journal of Clinical Otorhinolaryngology Head and Neck Surgery
Ano de publicação:
2013
Tipo de documento:
Artigo
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