Expression and methylation status of IGFBP-rP1 gene in laryngocarcinoma / 临床耳鼻咽喉头颈外科杂志
Journal of Clinical Otorhinolaryngology Head and Neck Surgery
; (24): 1352-1359, 2013.
Article
em Zh
| WPRIM
| ID: wpr-747116
Biblioteca responsável:
WPRO
ABSTRACT
OBJECTIVE@#To investigate the relationship between the promoter methylation and protein expression of insulin-like growth factor binding protein-related protein 1(IGFBP-rP1) in laryngeal squamous cell carcinoma (LSCC).@*METHOD@#Methylation specific PCR (MSP) approach and immunohistochemistry methods were used to examine the methylation status and protein expression of IGFBP-rP1 in 45 samples of laryngeal carcinoma and 18 samples of tissues beside cancer.@*RESULT@#For the promoter site, methylation frequency of IGFBP-rP1 in tumor specimens (33.3%, 15/45) was significantly higher than that in corresponding normal tissues (5.6%, 1/18) (P < 0.05). The protein expression of IGFBP-rP1 in tumor tissues was significantly higher than that in corresponding normal tissues (P < 0.05) and was inversely correlated with its methylation status of promoter.@*CONCLUSION@#Epigenetic silencing of IGFBP-rP1 gene expression by promoter hypermethylation may play an important role in LSCC.
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Índice:
WPRIM
Assunto principal:
Patologia
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Adenocarcinoma
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Neoplasias Laríngeas
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Proteínas de Ligação a Fator de Crescimento Semelhante a Insulina
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Metilação de DNA
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Genética
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Metabolismo
Limite:
Aged
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Female
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Humans
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Male
Idioma:
Zh
Revista:
Journal of Clinical Otorhinolaryngology Head and Neck Surgery
Ano de publicação:
2013
Tipo de documento:
Article