Erythrocyte Depletion Effect of Compression Method with 6% Hydroxyethyl Starch in Human Umbilical Cord Blood / 대한수혈학회지
Korean Journal of Blood Transfusion
;
: 179-186, 1999.
Artigo
em Coreano
| WPRIM
| ID: wpr-74746
ABSTRACT
BACKGROUND:
Stem cell transplantation with high dose chemoradiotherapy has made it possible to cure a significant proportion of patients with hematopoietic malignancies. RBC purging is an essential step prior to transplantation in ABO mismatched setting. The purpose of this study is to develop a new simple method for RBC depletion from harvested stem cells.METHODS:
Ten cord bloods were collected during deliveries at Kyungpook National University Hospital, Taegu, Korea at August, 1998. All nucleated cells were collected after the compression of bags with 6% hydroxyethyl starch and cord blood for 4 hours. The cell viability of mononucleated cell (MNC) was calculated after 24~72 hours. RESLUTS The collected mean volume of cord blood was 37.1 +/- 6.7 ml. The WBC count was 10,852 +/- 1,137/microL. The 3.90 +/- 0.62x108 TNC and 1.82 +/- 0.36x108 MNC were obtained per collection. TNC efficiency was 83.8 +/- 7.01% and MNC efficiency was 90.9 +/- 9.23% by compression method with 6% hydroxyethyl starch. RBC contamination was negligible. The cell viabilities of mononuclear cell were kept in a range of 99~100% after 24~72 hours.CONCLUSION:
Because compression method with 6% hydroxyethyl starch progresses in closed system is simple & easy, has high erythrocyte depletion efficacy, and can maintain high viability of stem cells, it can be used for RBC purging in ABO mismatched stem cell transplantation.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Amido
/
Células-Tronco
/
Cordão Umbilical
/
Sobrevivência Celular
/
Neoplasias Hematológicas
/
Transplante de Células-Tronco
/
Eritrócitos
/
Sangue Fetal
/
Quimiorradioterapia
/
Coreia (Geográfico)
Limite:
Humanos
País/Região como assunto:
Ásia
Idioma:
Coreano
Revista:
Korean Journal of Blood Transfusion
Ano de publicação:
1999
Tipo de documento:
Artigo
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