Development and completion of the DNA extraction procedure to directly detect diarrheagenic Escherichia coli isolates
Journal of Medical Research
;
: 92-97, 2008.
Artigo
em Vietnamita
| WPRIM
| ID: wpr-748
ABSTRACT
Background:
It is important to diagnosis and properly treat patients with diarrhea is having a highly sensitive and specific technique to rapidly identify the caused bacteria, especially Diarrheagenic Escherichia Coli (DEC) isolates.Objectives:
To develop and complete the bacterial DNA extraction procedure and optimize the DNA concentration for multiplex PCR for DEC. Materials andmethod:
7 reference strains of DEC and 10 fecal samples taken randomly were tested using DNA extraction and PCR techniques. Results. A bacterial DNA extraction procedure has been developed and optimized. This is a simple process and does not require expensive equipment. The test result is available after 90 \u2013 100 minutes. The minimum DNA content required for PCR to give positive results is 100ng per reaction.Conclusion:
The development and completion of DNA extraction procedure plays an important role in early detection of DEC in fecal samples and serves as a base for further research on diarrheagenic bacteria.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Reação em Cadeia da Polimerase
/
Escherichia coli
Tipo de estudo:
Estudo de rastreamento
Idioma:
Vietnamita
Revista:
Journal of Medical Research
Ano de publicação:
2008
Tipo de documento:
Artigo
Similares
MEDLINE
...
LILACS
LIS