Inhibition of growth and proliferation of Hep-2 cells by targeting hTERT gene using small interference RNA technology / 临床耳鼻咽喉头颈外科杂志

ABSTRACT

OBJECTIVE@#To investigate the inhibition of hTERT gene to Hep-2 cells of laryngeal neoplasms.@*METHOD@#siRNA-hTERT mRNA was designed. Hep-2 cells, cultured in vitro, were transfected with lipofectamine 2000 and the inhibitory effect was detected by reverse transcriptase (RT-PCR), MTT assay, and morphology.@*RESULT@#1. siRNA were transfected into Hep-2 cells successfully; 2. RT-PCR showed the exogenous hTERT was knocked down 70% by siRNA; 3. The morphology and the MTT result showed the hTERT siRNA to be able effectively to suppress the Hep-2 cell multiplication, the suppression rate achieves 65%.@*CONCLUSION@#siRNA target to hTERT can remarkably suppress the proliferation of Hep-2 cell.