Elemene in the treatment of the transplantable model of human laryngeal squamous carcinoma in nude mice / 临床耳鼻咽喉头颈外科杂志
Journal of Clinical Otorhinolaryngology Head and Neck Surgery
;
(24): 417-420, 2007.
Artigo
em Chinês
| WPRIM
| ID: wpr-748405
ABSTRACT
OBJECTIVE@#To investigate the inhibitory effect of elemene on the tumor growth of human laryngeal squamous carcinoma (LSCC) and to explore the possible mechanism of its inhibitory effect on LSCC.@*METHOD@#Nude mice model engrafted with laryngocarcinoma was established by using human laryngeal squamous carcinoma cell line (Hep-2). When the transplantable tumor size is more than 10 mm, the mice were given elemene (120 mg/kg) in the treatment group or PBS in the control group, at the day of 1, 4, 7, 10, 13, 16, 19 and 22 respectively. The animals were killed three days after the last treatment. The tumor volume, tumor weight,mice net weight and tumor inhibition rate were measured. The expression of vascular endothelial growth factor-C (VEGF-C) and vascular endothelial growth factor receptor-3 (VEGFR-3) mRNA were quantitated by using reverse transcription and polymerase chain reaction technique.@*RESULT@#Elemene could inhibit the tumor growth in vivo. The differences were statistically significant for the mice net weight, tumor weight, and tumor volume between the treatment group and the control group. The tumor inhibition percentage was 52.24%. The gene expression of VEGF-C and VEGFR-3 of the treatment group is lower than that of the control group and the differences were statistically significant (P < 0.01).@*CONCLUSION@#Elemene could inhibit the subcutaneous plantation of human laryngeal carcinoma in nude mice and its mechanism may be associated with inhibited expression of VEGF-C and VEGFR-3.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Sesquiterpenos
/
Medicamentos de Ervas Chinesas
/
Carcinoma de Células Escamosas
/
Neoplasias Laríngeas
/
Ensaios Antitumorais Modelo de Xenoenxerto
/
Receptor 3 de Fatores de Crescimento do Endotélio Vascular
/
Linhagem Celular Tumoral
/
Fator C de Crescimento do Endotélio Vascular
/
Usos Terapêuticos
/
Tratamento Farmacológico
Tipo de estudo:
Estudo prognóstico
Limite:
Animais
/
Feminino
/
Humanos
/
Masculino
Idioma:
Chinês
Revista:
Journal of Clinical Otorhinolaryngology Head and Neck Surgery
Ano de publicação:
2007
Tipo de documento:
Artigo
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