Screening and expression of tumor markers in laryngeal carcinoma / 临床耳鼻咽喉头颈外科杂志
Journal of Clinical Otorhinolaryngology Head and Neck Surgery
;
(24): 409-412, 2011.
Artigo
em Chinês
| WPRIM
| ID: wpr-748452
ABSTRACT
OBJECTIVE@#To select relatively specific biomarkers in serum from laryngeal carcinoma patients using surface-enhanced laser desorption/ionization time-of-flight mass spectrometry (SELDI-TOF-MS) technology.@*METHOD@#The serum samples of 32 cases of laryngeal carcinoma preoperation and 15 cases of laryngeal carcinoma 10 days postoperation and 40 healthy controls on IMAC-Cu protein chip were collected. The spectrometric protein profiling was detected by SELDI-TOF-MS, the data was analyzed by Biomarker Wizard software and Biomarker Pattern software.@*RESULT@#Twenty-six differentially expressed proteins in serum were screened by analysis of protein spectra of preoperative and postoperative patients and healthy controls. Eight proteins (4048.09, 5073.79, 5339.29, 3795.38, 9187.24, 15323.8, 6111.92, 6943.87) were obtained for making up patterns that was able to class the preoperative-team and postoperative-team. Corresponding correct ratio were 75% (30/40), 71.88% (23/32) and 66.67% (10/15).@*CONCLUSION@#SELDI-TOF-MS technology is a quick, easy, convenient and high output analyzing method that it is capable of selecting several relatively specific, potential biomarkers from the serum of laryngeal carcinoma patients and may have clinic value.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Sangue
/
Biomarcadores Tumorais
/
Estudos de Casos e Controles
/
Neoplasias Laríngeas
/
Espectrometria de Massas por Ionização e Dessorção a Laser Assistida por Matriz
/
Análise Serial de Proteínas
/
Métodos
Tipo de estudo:
Estudo diagnóstico
/
Estudo observacional
/
Estudo de rastreamento
Limite:
Idoso
/
Feminino
/
Humanos
/
Masculino
Idioma:
Chinês
Revista:
Journal of Clinical Otorhinolaryngology Head and Neck Surgery
Ano de publicação:
2011
Tipo de documento:
Artigo
Similares
MEDLINE
...
LILACS
LIS