The auditory study of guinea pig immunized with P0 protein / 临床耳鼻咽喉头颈外科杂志

ABSTRACT

OBJECTIVE@#To investigate the auditory characteristics of guinea pigs immunized with purified P0 protein from inner ear of guinea pigs.@*METHOD@#Purified inner ear P0 protein was made by Preparative SDS-PAGE. To evaluate the results of Auditory Brainstem Response (ABR), Compound Action Potention (CAP), and Distortion Product Oto-acoustic Emissions (DPOAE) in guinea pigs immunized with purified guinea pigs inner ear myelin protein P0.@*RESULT@#Seven ears of the guinea pigs immunized with purified inner ear P0 protein developed hearing loss. In the ABR study, peak latencies of wave I, III and the interpeak latency of I -III, I-IV were elevated in this group of guinea pigs compared with the control group (P 0.05). Even though the contra-lateral suppressive effect had the depressed tendency, but there was no significant different when compared with the controls (P > 0.05).@*CONCLUSION@#Purified inner ear P0 protein is an important autoimmune inner ear antigen and can develop autoimmune disease of the auditory nerve.