The expression of COX-2 mRNA and protein in human laryngeal squamous cell carcinoma / 临床耳鼻咽喉头颈外科杂志

ABSTRACT

OBJECTIVE@#To investigate the expression of COX-2 in both molecule and protein levels in laryngeal squamous cell carcinoma and the relationship between its intensity and histological grade, clinical stage and lymphoma metastasis.@*METHOD@#By using emi-quantify RT-PCR and Immunohistochemistry, the expression of the COX-2 mRNA and COX-2 protein in laryngeal squamous cell carcinoma and polyp of vocal cord were examined.@*RESULT@#Compared with the polyp of vocal cord tissue, the expression level of COX-2 mRNA and COX-2 protein in human laryngeal squamous cell carcinoma were significantly increased (P < 0.01). The intensity of COX-2 mRNA and protein expression increased as the laryngeal squamous cell carcinoma progresses developed.@*CONCLUSION@#COX-2 is high expressed in human laryngeal squamous cell carcinoma, and it may play an significant role in the growth, invasion and metastasis of human laryngeal squamous cell carcinoma. A combination of emi-quantify RT-PCR and immunohistochemistry is sensitive and specific for laryngeal neoplasms detection.