Effects of siRNA targeting NF-kappaBp65 against in proliferation of Hep-2 cells and the expression of mRNA and protein of NF-kappaBp65 / 临床耳鼻咽喉头颈外科杂志
Journal of Clinical Otorhinolaryngology Head and Neck Surgery
;
(24): 416-418, 2008.
Artigo
em Chinês
| WPRIM
| ID: wpr-749056
ABSTRACT
OBJECTIVE@#To study siRNA inhibited the expression of mRNA and protein of NF-kappaBp65 in the Hep -2 cell line.@*METHOD@#Hep-2 were transfected with p65SiRNA. Western blotting was used to examine the protein levels of NF-kappaBp65. RT-PCR method was adopted to determine the mRNA expression of NF-kappaBp65. MTT method was adopted to investigate the proliferation of the Hep-2 cells after the transfection of p65siRNA.@*RESULT@#The western blotting result showed that the level of NF-kappaBp65 protein was gradually declined after transfection of p65siRNA. The RT-PCR result showed that transfection with p65siRNA caused special degradation of the p65mRNA in Hep-2 cells at 24, 48 and 72 h. After transfection with p65siRNA.@*CONCLUSION@#p65siRNA has significant inhibition effects on the proliferation of the Hep-2 cells and expression of purpose gene mRNA and protein. The inhibition effects are time depended.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
RNA Mensageiro
/
RNA Interferente Pequeno
/
Linhagem Celular Tumoral
/
Proliferação de Células
/
Fator de Transcrição RelA
/
Genética
Limite:
Humanos
Idioma:
Chinês
Revista:
Journal of Clinical Otorhinolaryngology Head and Neck Surgery
Ano de publicação:
2008
Tipo de documento:
Artigo
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