Effect of interferon-gamma on the expression of vascular endothelial growth factor C on Hep-2 laryngeal carcinoma cell lines / 临床耳鼻咽喉头颈外科杂志

ABSTRACT

OBJECTIVE@#To observe the effect of interferon-gamma on the expression of vascular endothelial growth factor C on Hep-2 laryngeal carcinoma cell lines.@*METHOD@#Hep-2 cell was exposed to interferon-gamma at a concentration (10(3), 10(4), 10(5), 10(6), 10(7) U/L) for 0, 12, 24, 36, 48, 60, 72 h hours. MTT colorimetric assay was used to evaluate the effect of interferon-gamma on Hep-2 cell proliferation after incubation with interferon-gamma. Hep-2 cell was harvested to be detected VEGF-C mRNA levels by realtime polymerase chain reaction (Realtime-PCR) with interferon-gamma (10(6) U/L) for 0, 2, 6, 12, 24, 36, 48, 60, 72 h hours. The VEGF-C protein level in supernatants was determined by enzyme-linked immunosorbent assay (ELISA) and in cytoplasma by immunocytochemical staining and Western blot analysis with the same drug treatment as Realtime-PCR.@*RESULT@#Hep-2 cell was significantly inhibited by IFN-gamma at high concentration. Expression of VEGF-C mRNA and protein levels of Hep-2 cell at 10(6) U/L concentrations of interferon-gamma were lower than that of normal control (P < 0.05) at 48 h.@*CONCLUSION@#IFN-gamma can inhibit the expression of VEGF-C in Hep-2 cell with the level of mRNA and protein.