Induction of Apoptosis and Inhibition of Cellular Proliferation in Aspirin-treated SNU-668 Human Gastric Adenocarcinoma Cell Lines / 대한암학회지

ABSTRACT

PURPOSE: The aims of this study was to examine the anti-proliferative effect and apoptosis induction by aspirin using SNU-668 human gastric adenocarcinoma cell lines. MATERIALS AND METHODS: After treating SNU-668 cell lines with various concentrations of aspirin, cell growth was quantified by MTT assay. Apoptosis was determined by comparing aspirin treated cell lines by immunofluores cence with control cells after Hoechst 33258 staining. Cell lines were further examined using ELISA. Cell cycle was evaluated by FACS. RESULTS: Inhibition of cellular proliferation occurred when cells were treated with aspirin at concetrations of 1 mM or more. Aspirin also induced apoptosis =in these cell lines. Percentages of induction were 3.0+/- 0.6% , 4.8, +/- 0.6%, 17.5+/-0.8%, and 19.2+/-0.7% at 0, 0.5, 1 and 2 mM concentration of aspirin, respectively. ELISA confirmed apoptosis in these cells. However, cell cycle was not affected. CONCLUSION: These results indicate that induction of apoptotic cell death contribute to the anti-proliferative effect of aspirin on SNU-668 human gastric adenocarcinoma cell lines without affecting cell cycle. These findings suggest aspirin may play an important role in cancer prevention and tumor regression in humans.