Construction of nasopharyngeal carcinoma CNE-2 cell lines expressing stable fusion suicide gene CD/UPRT. UL49 / 临床耳鼻咽喉头颈外科杂志
Journal of Clinical Otorhinolaryngology Head and Neck Surgery
;
(24): 178-181, 2014.
Artigo
em Chinês
| WPRIM
| ID: wpr-749401
ABSTRACT
OBJECTIVE@#To construct nasopharyngeal carcinoma CNE-2 cell lines expressing stable fusion suicide gene CD/UPRT. UL49.@*METHOD@#The plasmids of pcDNA3.1 (-)E6. BARF1p. CD/UPRT. UL49 was transfected into CNE-2 cells through lipofectamine, and the transfected CNE-2 cells were selected by G418 and prodrugs for getting the cells expressing fusion CD/UPRT. UL49 gene. The protein produced by the suicide gene was tested by Western-blotting in CNE-2 cells.@*RESULT@#Suicide genes were expressed stably in CNE-2 cells.@*CONCLUSION@#We constructed nasopharyngeal carcinoma cell lines CNE-2 expressing stable suicide gene through lipofectamine.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Carcinoma
/
Neoplasias Nasofaríngeas
/
Fusão Gênica Artificial
/
Linhagem Celular Tumoral
/
Genes Transgênicos Suicidas
/
Carcinoma Nasofaríngeo
/
Genética
/
Métodos
Limite:
Humanos
Idioma:
Chinês
Revista:
Journal of Clinical Otorhinolaryngology Head and Neck Surgery
Ano de publicação:
2014
Tipo de documento:
Artigo
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