Effects of IAA/HRP on the proliferation and apoptosis of SACC-83 cells / 临床耳鼻咽喉头颈外科杂志

ABSTRACT

OBJECTIVE@#To study the effect of IAA/HRP on the proliferation and apoptosis of human SACC-83 cells in vitro,and its molecular mechanism.@*METHOD@#The rate of proliferation inhibition was determined by CCK-8 assay, the apoptosis-related gene Caspase-3 and Livinα mRNA and protein expression levels were measured by real-time PCR and Western blot.@*RESULT@#IAA/HRP could inhibit proliferation of SACC-83, which depending on the time and dosage (P < 0.05); can-up-regulate the Caspase-3 mRNA and protein expression levels and reduction of the mRNA and protein of the Livina expression, (both P < 0.05).@*CONCLUSION@#IAA/HRP can inhibit the proliferation and induce the apoptosis of SACC-83 cells, which may due to its regulation of the expression of Caspase-3 and Livinα mRNA expression.