Imidacloprid inhibits IgE-mediated RBL-2H3 cell degranulation and passive cutaneous anaphylaxis
Asia Pacific Allergy
; (4): 236-244, 2016.
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| ID: wpr-750078
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ABSTRACT
BACKGROUND: Imidacloprid has been commonly used as a pesticide for crop protection and acts as nicotinic acetylcholine receptor agonists. Little information about the relationship between imidacloprid and allergy is available. OBJECTIVE: This study aims to examine the effects of imidacoprid on IgE-mediated mast cell activation. METHODS: The rat basophilic leukemia cell line RBL-2H3 (RBL-2H3 cells) were treated with 10⁻³ – 10⁻¹² mol/L imidacloprid, followed by measuring the mediator production, influx of Ca²⁺ in IgE-activated RBL-2H3 cells, and the possible effects of imidacoprid on anti-dinitrophenyl IgE-induced passive cutaneous anaphylaxis (PCA). RESULTS: It was shown that imidacoprid suppressed the production of histamine, β-hexosaminidase, leukotriene C4, interleukin-6, tumor necrosis factor-α, and Ca²⁺ mobilization in IgE-activated RBL-2H3 cells and decreased vascular extravasation in IgE-induced PCA. CONCLUSION: It is the first time to show that imidacloprid suppressed the activation of RBL-2H3 cells.
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Assunto principal:
Basófilos
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Anafilaxia Cutânea Passiva
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Leucemia
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Histamina
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Degranulação Celular
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Linhagem Celular
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Interleucina-6
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Receptores Nicotínicos
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Leucotrieno C4
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Proteção de Cultivos
Limite:
Animals
Idioma:
En
Revista:
Asia Pacific Allergy
Ano de publicação:
2016
Tipo de documento:
Article