Major house dust mite allergen, Der p I, activates phospholipase D in human peripheral blood mononuclear cells from allergic patients: involvement of protein kinase C
Experimental & Molecular Medicine
;
: 67-71, 2000.
Artigo
em Inglês
| WPRIM
| ID: wpr-75102
ABSTRACT
The major house-dust-mite allergen, Der p I, stimulates the phospholipase D (PLD) in peripheral blood mononuclear cells (PBMC) from allergic patients with maximal responses after 30 min exposure. At 30 min, Der p I stimulated PLD activity by 1.4-fold in mild, 1.6-fold in moderate and 2-fold in severe allergic patients over control values (p < 0.05). When the cells were pretreated for 24 h with phorbol myristate acetate to down-regulate protein kinase C (PKC), PLD stimulation by Der p I was largely abolished. These results indicate that in PBMC from allergic patients, Der p I can stimulate PLD activity, and that PKC activation is involved in this stimulation.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Fosfolipase D
/
Técnicas In Vitro
/
Proteína Quinase C
/
Imunoglobulina E
/
Testes Cutâneos
/
Acetato de Tetradecanoilforbol
/
Leucócitos Mononucleares
/
Alérgenos
/
Glicoproteínas
/
Regulação para Baixo
Limite:
Adulto
/
Humanos
Idioma:
Inglês
Revista:
Experimental & Molecular Medicine
Ano de publicação:
2000
Tipo de documento:
Artigo
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