The correlation between CB2R and autophagy-related protein LC3 expression in the hippocampal CA1 re-gion of the model with status epilepticus / 国际儿科学杂志

ABSTRACT

Objective The expression and correlation between cannabinoid receptor type 2 ( CB2R) and autophagy-related protein microtubule-associated protein 1 light chain 3 (LC3) in the hippocampal CA1 re-gion of developing rats with status epilepticus ( SE) were investigated. Methods SE model was established u-sing lithium chloride-pilocarpine intraperitoneally in Sprague-Dawley ( SD) rats and all the rats were randomly divided into four groups ( control group and 3h, 24h, 3d groups after SE). The expressions of CB2R and LC3 in the hippocampal CA1 region at different times were observed using double-label immunofluorescence and Western blotting. Spearman correlational analysis was used to compare the relationship between the two factors. Results Immunofluorescence showed that the expression of CB2R was up-regulated dynamically and peaked at 24h and presented parabolic changes over time. The expression of LC3 changed in accordance with CB2R and e-ven co-expressed with CB2R partly, especially on neurons. Western blotting results furtherly showed the simi-larity of the expression of CB2R and LC3, and finally Spearman correlational analysis presented the significant correlation between these two factors (r=0. 7161, P<0. 05). Conclusion Significant correlation exists be-tween the expression of CB2R and LC3 in the hippocampal CA1 neurons of developing rats with SE, indicating the essential role of CB2R in autophagy regulation of hippocampal CA1 neurons.