JNK and Akt genes in pancreatic tissue of diabetic rats and the effects of protein expression and phosphorylation modification / 国际中医中药杂志

ABSTRACT

Objective To observe the effects of Yutang pill on pancreatic tissue, JNK, Akt gene, protein expression and phosphorylation of diabetic rats. Methods A total of 150 male SD rats were divided into blank group, model group, western medicine control group, Chinese medicine control group, Yutang Pill low dose group and Yutang pill high dose group according to random number table, 20 rats in each group. In addition to the blank group, the other groups of rats were prepared for the diabetes model. The western medicine control group was intragastrically administered with metformin hydrochloride suspension 0.09 g/kg, the Chinese medicine control group was administered with the suspension of glycoside tablets 2.43 g/kg, and the low-dose and high-dose groups of Yutang pills were respectively administered with Yutang pills 1.35 and 2.70 g/kg. The blank group and the model group were intragastrically administered with equal volume of saline. 1 time/d, 8 weeks. After continuous administration, the material was taken. The expression levels of JNK and Akt in pancreatic tissue were detected by Real-time PCR. The expression of JNK and Akt protein and phosphorylation in pancreatic tissue were detected by Western blot. Results Compared with the model group, the expression of JNK mRNA (0.57 ± 0.07, 0.95 ± 0.13 vs. 1.14 ± 0.19) and P-JNK/JNK (0.222 ± 0.038, 0.817 ± 0.104 vs. 1.140 ± 0.136) in the low- and high dose group significantly decreased (P<0.01). The expression of Akt mRNA (2.09 ± 0.13 vs. 1.63 ± 0.12) and P-Akt(Ser473)/Akt (0.385 ± 0.072 vs. 0.130 ± 0.027) significantly increased (P<0.01). Conclusions The Yutang pill can reduce the expression of JNKmRNA in pancreatic tissue of T2DM rats and inhibit the phosphorylation of JNK protein. The expression of AktmRNA increased and the phosphorylation level of Akt protein increased.