Effect of hyaluronidase on expression of neuron＿glial antigen 2 and myelin basic protein after germinal ma-trix＿intraventricular hemorrhage in premature rabbits / 中华实用儿科临床杂志

ABSTRACT

Objective To investigate the neuroprotective effect of hyaluronidase( HAase)on rabbit brain tissue in germinal matrix-intraventricular hemorrhage( GM -IVH)premature rabbits. Methods Eighty premature rabbits of gestation age 29 days were randomly divided into normal group,GM-IVH control group and HAase treatment group. The rabbits in GM -IVH control group and HAase treatment group were given intraperitoneal injection of 50 g/L glycerol to establish GM-IVH animal model,while the premature rabbits in normal group were given the same dose of 9 g/L saline. The GM-IVH was screened by using cranial ultrasound. The premature rabbits in HAase treat-ment group were given HAase into the lateral forebrain while the rabbits in the GM-IVH control group were given the same dose of 9 g/L saline. In 3 days,7 days and 14 days after birth,the premature rabbits were killed and brain tissue were separated. The expression of neuron - glial antigen 2( NG2 )were detected by adopting immunohistochemical method. The expression of myelin basic protei(n MBP)were detected by Western blot method. Results The expression of NG2 in 3 groups of premature rabbits decreased gradually with the increase of ag[e the values of NG2 in the normal group were(62. 65 ± 33. 58)×104(,15. 61 ± 4. 22)×104 ,and(13. 54 ± 4. 51)×104 on the 3rd ,7th ,and 14th day;those in the GM-IVH control group were(54. 58 ± 25. 48)×104 ,(48. 91 ± 22. 49)×104 ,(7. 18 ± 2. 28)×104 on the 3rd ,7th , and 14th day;those in the HAase treatment group were(148. 13 ± 27. 30)×104 ,(88. 38 ± 14. 55)×104 ,(77. 98 ± 18. 96)×104 on the 3rd,7th,and 14th day]. At the same time,the expression of NG2 in the HAase treatment group was higher than that of the GM-IVH control group,and the differences were statistically significant among the 3 groups at any time(all P<0. 05). The expression of MBP protein in the 3 groups increased with the increase of day age[MBP protein in the normal group were(0. 30 ± 0. 22)×103 ,(1. 91 ± 0. 43)×103 ,and(5. 67 ± 2. 14)×103 on the 3rd , 7th,and 14th day;those in the GM-IVH control group were(0. 87 ± 0. 12)×103,(1. 15 ± 0. 22)×103 and(2. 54 ± 0. 69)×103 on the 3rd,7th,and 14th day;those in the HAase treatment group were(0. 91 ± 1. 01)×103 ,(2. 25 ± 0. 66)×103 ,(3. 40 ± 1. 10)×103 on the 3rd,7th ,and 14th day]. The difference was statistically significant between the normal group and the HAase treatment grou(p P<0. 05). Conclusions After HAase treatment,the expression of NG2 and MBP can be improved to a certain extent,promoting the development of oligodendrocytes and regeneration of myelin sheath.